Noi tehnici de detectare a cancerului de col uterin

In dorinta de a ne alinia la cele mai noi tehnici si mijloace de detectare in cadrul metodelor de screening pentru cancerul de col uterin va informam ca,  in cadrul laboratoarelor Clinica Sante,  alaturi de detectie ADN a HPV (Papiloma Virus Uman) avem posibilitatea testarii in scopul detectiei ARN mesager pentru oncogenele E6/E7 ale genotipurilor HPV cu risc inalt (cea mai specifica metoda de screening pentru cancerul de col uterin).

Cancerul de col uterin este cauzat in 99.7 % din cazuri de infectia persistenta cu HPV.  Aproximativ 80 % din infectiile cu HPV regreseaza simptomatic sau asimptomatic. Pentru progresia spre cancerul de col uterin este necesara persistenta infectiei cu HPV si integrarea acestuia in genomul celulei gazda.  Din momentul integrarii se exprima activitatea oncogenelor E6/E7, iar probabilitatea regresiei infectiei cu HPV este mult mai mica.

Avantajul metodei ARN fata de ADN in diagnosticarea infectiilor cu HPV este specificitatea mult mai mare. Probabilitatea ca o femeie cu test ARN pentru HPV pozitiv sa fie diagnosticata histologic cu neoplasm intraepitelial cervical (CIN 2+) este de 70 de ori mai mare decat la o femeie cu acelasi test negativ. Pentru testele ADN de HPV aceeasi probabilitate este doar de 6 ori mai mare.

In cazul femeilor sub 30 de ani, unde semnificatia clinica a tehnicilor ADN este scazuta datorita frecventelor infectii tranzitorii,  metoda ARN mesager este singura metoda cu reala utilitate clinica pe acest segment de populatie.

In cadrul  Departamentul de Anatomie Patologica al  Clinicii Sante Bucuresti se poate realiza analiza histopatologica a materialului biopsic.

Se pot procesa piese histologice diverse, de la biopsii (sfera ginecologica, gastrica, urologica, ORL,etc.) pana la piese chirugicale complexe, rezultatele histopatologice putand fi  eliberate in termen de 7 zile lucratoare, astfel:

Piesa histologica mica  (1-2 blocuri de parafina)  Ex: biopsie col, polip col,  chiuretaj uterin, bio gastric, bio vezica, colecist,  etc.

Piesa histologica medie (3-5 blocuri de parafina)  Ex: Piesa de histerectomie interanexiala , lipom voluminos, amputarie col lezional, etc

Piesa histologica complexa  ( mai mult de 5 blocuri de parafin a)  Ex: Histerectomie totala cu anexectomie bilaterala, piesa de colectomie, piesa de rezectie gastrica, etc.

Pretul analizelor histopatologice este unul in functie de marimea si complexitatea pieselor histologice.In the desire to align with the latest techniques and means of detection in screening methods for cervical cancer, we inform you that, in clinical laboratories Sante, DNA detection with HPV (human papilloma virus) we can test the for detection of E6/E7 oncogenes messenger RNA for high-risk HPV genotypes (the most specific method of screening for cervical cancer).

Cervical cancer is caused in 99.7% of cases of persistent infection with HPV. Approximately 80% of HPV infections regress symptomatic or asymptomatic. For progression to cervical cancer is persistent HPV infection required and its integration into the host cell genome. Since integration is expressed E6/E7 oncogenes activity and regression of HPV infection probability is much lower.

The method of RNA to DNA in diagnosing HPV infections is much higher specificity. The probability that a woman with a positive test for HPV RNA is diagnosed histologically with cervical intraepithelial neoplasia (CIN 2 +) is 70 times greater than a woman with the same negative test. HPV DNA tests as likely is only 6 times.

For women under 30, where the clinical significance of DNA techniques is low due to frequent transient infections, messenger RNA method is the only method with real clinical utility in this segment of the population.

In the Department of Pathology of Bucharest Sante Clinic can be Histopathological analysis of biopsy material.

It can process different histological parts from biopsies (gynecologic, gastric, urology, ENT, etc..) To complex surgical parts, histopathological findings can be issued within 7 working days, as follows:

Histologic small piece (1-2 blocks of paraffin) Ex: cervical biopsy, cervical polyps, uterine curettage, gastric bio, bio bladder, gallbladder, etc..

Average histologic piece (3-5 blocks of paraffin) Ex: Part of hysterectomy interanexiala, lipoma bulky cervical lesion amputations, etc.

Complex histologic play (more than 5 Paraffin blocks a) Ex: anexectomy total hysterectomy with bilateral track colectomy, gastric resection piece, etc..

Price histopathological analysis is based on the size and complexity of histological parts.